Application

ELISA Analysis: A representative lot detected 8-Nitroguanine in ELISA applications (Sawa, T., et. al. (2007). Nat Chem Biol. 3(11):727-35; Yoshitake, J., et. al. (2004). J Virol. 78(16):8709-19).

Immunocytochemistry Analysis: A representative lot detected 8-Nitroguanine in Immunocytochemistry applications (Ihara, H., et. al. (2011). Nitric Oxide. 25(2):169-75; Sawa, T., et. al. (2007). Nat Chem Biol. 3(11):727-35; Yoshitake, J., et. al. (2004). J Virol. 78(16):8709-19).

Anti-8-Nitroguanine, clone NO2-52, Cat. No. MABN2411, is a highly specific mouse monoclonal antibody that targets 8-Nitroguanine and has been tested for use in ELISA, Immunocytochemistry, and Western Blotting.

General description

8-Nitroguanosine, a highly reactive redox-active nucleic acid derivative, is formed by the action of peroxynitrite. It is a nitrated base of DNA and RNA formed as a result of chronic inflammation. It has been well established that large amount of nitric oxide molecules and superoxide anion, generated as a result of inflammatory response, can cause nitration of guanosine. Such chemically modified nucleotides increase the incidence of mutation during DNA replication. 8-nitroguanosine is considered as a marker of DNA damage related to mutation and cancer. Nitrated guanine nucleosides and nucleotides in the nucleotide pool are shown to contribute to oxidative stress via production of superoxide mediated by various reductases and may disturb or modulate directly various important proteins and enzymes, such as G-proteins and cGMP-dependent enzymes. Clone NO2-52 is a highly specific monoclonal antibody that recognizes 8-nitroguanine and 8-nitroguanosine. However, it does not cross-react with normal nucleotide bases. (Ref.: Ohshima, H., et al. (2006). Antioxid. Redox Signal 8(5-6); 1033-45; Sawa, T., et al. (2007). Nat. Chem. Biol. 3(11); 727-35).

Immunogen

BSA conjugated 8-nitroguanosine.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blottingwith Nitroguanine-conjugated BSA (NG-BSA).

Western Blotting Analysis: 1 µg/mL of this antibody detected 8-Nitroguanine in Nitroguanine-conjugated BSA (NG-BSA). It does not react with BSA alone.

Specificity

Clone NO2-52 specifically detects 8-nitroguanine.